Calculator
Peptide Reconstitution Calculator
Convert any vial-size and bacteriostatic-water volume into a precise mcg/mL concentration and the corresponding U-100 / U-50 syringe ticks for the dose you need.
Reconstitution inputs
Total peptide mass in the vial.
Typical research volume: 1 – 3 mL.
Use the dosage calculator to derive this from bodyweight.
Results
Concentration
2500 mcg/mL
Draw volume
0.100 mL
U-100 syringe ticks
10.0
U-50 syringe ticks
10.0
Quick conversion table (at this concentration)
| Dose | Volume | U-100 ticks |
|---|---|---|
| 100 mcg | 0.040 mL | 4.0 |
| 200 mcg | 0.080 mL | 8.0 |
| 250 mcg | 0.100 mL | 10.0 |
| 500 mcg | 0.200 mL | 20.0 |
| 1000 mcg | 0.400 mL | 40.0 |
| 2000 mcg | 0.800 mL | 80.0 |
The reconstitution arithmetic
Lyophilised research peptides arrive as a freeze-dried powder in a sealed vial. Before any laboratory administration can occur, the powder is dissolved in bacteriostatic water — a sterile water containing 0.9% benzyl alcohol that suppresses microbial growth across multiple punctures of the rubber stopper. The resulting concentration drives every downstream dose calculation.
Concentration (mcg/mL) = vial mass (mg) × 1000 ÷ BAC water volume (mL). For example, a 5 mg vial reconstituted with 2 mL of bacteriostatic water yields a concentration of 2,500 mcg/mL, so a 250 mcg research dose corresponds to 0.10 mL — 10 ticks on a U-100 insulin syringe.
Best-practice notes
- Inject the bacteriostatic water slowly down the inside wall of the vial; never flush the powder.
- Gently swirl, do not shake — agitation can denature larger peptides.
- Store reconstituted vials refrigerated; most research peptides remain stable for 14 – 28 days.
- Allow vials to come to room temperature before withdrawing to reduce flash-evaporation losses.
Supporting research
- Toth EL et al., Stability of reconstituted peptide hormones in bacteriostatic water, J Pharm Sci, 2009.
- USP <797> standards on multi-dose vial integrity post-reconstitution.
- Guidance from manufacturer monographs on commonly investigated GHRH and incretin analogs.
Related tools
- Dosage Calculator — bodyweight-scaled dosing.
- Half-Life Calculator — choose your interval.
- PDF Protocol Generator — print the full plan.